Journal: PLoS ONE

Article Title: Platelet Adhesion and Degranulation Induce Pro-Survival and Pro-Angiogenic Signalling in Ovarian Cancer Cells

doi: 10.1371/journal.pone.0026125

Figure Lengend Snippet: [ A ] Platelet adhesion to ovarian cancer cells was quantified based on the fluorescence detection of labelled platelets. Platelet adhesion to fibrinogen and BSA were used as ± controls [n = 8 + SEM, * = p<0.05 vs. BSA]. Fluorescence microscopy images of platelets adhering to A2780 [ B ] and 59M [ C ] cells under static conditions [representative of n = 3]. Ovarian cancer cells and platelets were stained for actin [green], platelets were stained specifically for CD42a [red/yellow].

Article Snippet: Phycoerythrin [PE]-labelled anti human P-selectin [mouse IgG], PE-labelled mouse IgG isotype control, and PE-labelled anti human CD42a [mouse IgG] antibodies were purchased from BD Pharmingen [San Diego, CA, USA].

Techniques: Fluorescence, Microscopy, Staining

Journal: Critical Care

Article Title: HES 130/0.4 impairs haemostasis and stimulates pro-inflammatory blood platelet function

doi: 10.1186/cc8223

Figure Lengend Snippet: Effects of hydroxyethyl starch (HES) 130/0.4 and HES 200/0.5 on platelet activation markers and formation of platelet-leukocyte conjugates. After haemodilution of 10% (white) or 40% (grey) with either HES solution or saline (Con), platelets were activated by adenosine diphosphate (ADP) (5 μM) or thrombin receptor-activating peptide (TRAP) (25 μM) for 5 minutes or remained inactivated (Basal). CD62P expression (a) and fibrinogen binding to platelets (b) as well as platelet-neutrophil (P-N) (c) and platelet-monocyte (P-M) (d) conjugates were determined by flow cytometry. The results are expressed as the relative numbers of platelets or leukocytes that were positive for CD62P or fibrinogen or the platelet-specific antigen CD42a. Data are given as mean ± standard error of the mean obtained from eight identical experiments. Significant differences between saline and HES 130/0.4 or HES 200/0.5: * P < 0.05, ** P < 0.005, *** P < 0.001. Significant differences between HES 130/0.4 and HES 200/0.5: § P < 0.05, §§ P < 0.01.

Article Snippet: After the samples were incubated for 5 minutes at 37°C, aliquots were mixed with anti-CD42a-PE and one of the following fluorescence-labelled antibodies: anti-CD62P-FITC, anti-CD45-FITC (both from Becton Dickinson, Heidelberg, Germany) or anti-fibrinogen-FITC (WAK-Chemie, Bad Sodenam Taunus, Germany; all antibody dilutions were 5 μL/100 μL).

Techniques: Activation Assay, Expressing, Binding Assay, Flow Cytometry